subculture cell meaning in English
传代培养细胞
Examples
- For the cryogenic preservation of fish , in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp , the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture , the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made
本文主要从鱼类种质保存的目的出发，一方面以四倍体鲫鲤鱼为材料，对四倍体鲫鲤鱼肾脏组织进行初步培养，为建立相应细胞库及下一步培养冻存的鱼类胚胎细胞奠定基础；另一方面，以鱼类组织细胞培养技术为基础，泥鳅胚胎细胞为材料，对鱼类胚胎细胞冻存培养方法进行初步研究，并应用该技术方法对草鱼早期胚胎细胞进行冻存培养实验。报告如下：本文用组织贴壁法对四倍体鲫鲤鱼肾组织进行原代、传代培养。 - The results showed that : when cultured in the medium of m199 , supplemented with 20 % bovine serum containing a moderate amount of antibiotics , the incubtion ph 7 . 2 - 7 . 4 , the culture temperature 28 . the primary culture cells formed the dense single wall within three weeks , the generation time of the subculture cells was 5 - 6 days , most cultured cells were fibroblastic - like cells with few epithelial - like cells
研究初步表明：在m199培养基加入20小牛血清(常规量双抗) ， ph在7 . 2 - 7 . 4之间， 28的培养条件，四倍体鲫鲤鱼肾组织原代培养三周左右即可形成致密单层，传代细胞为5 - 6天左右传一次。培养细胞以成纤维样细胞为主，有少数上皮样细胞。